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FGF-2 Protein, Human, Recombinant  Cite:0    分享

货号： IM64377
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规格:
5μg 10μg 20μg 50μg 100μg
单位:
支
单价:￥1100.00
基本信息
产品简介
运输与保存
附图
注意事项
参考文献
基本信息
  • 产品名称
    FGF-2 Protein, Human, Recombinant
  • 别名
    HBGF-2, fibroblast growth factor 2 (basic), FGFB, FGF-2, BFGF
  • 种属
    Human
  • 表达系统
    E. coli
  • 标签
    Tag Free
  • 蛋白编号
    P09038-4
  • 蛋白构建
    A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro143-Ser288) was expressed with an additional Met at the N-terminus. Predicted N terminal: Met
  • 蛋白纯度
    ≥ 95 % as determined by SDS-PAGE
  • 蛋白性状
    Lyophilized powder
  • 分子量
    16.5 kDa (predicted)
  • 缓冲液
    Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
  • 复溶方法
    Reconstituted with sterile deionized water to 0.15 mg/mL.
  • 内毒素
    < 10 EU/mg of the protein.
  • 生物活性
    1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 , VEGFA , EGF . Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested) 2.Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.01-0.1 ng/mL.
  • 研究背景
    Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.bFGF/FGF2 Protein & Antibody Products
产品简介

FGF-2 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16.5 kDa and the accession number is P09038-4.

运输与保存
  • Shipping
    In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice
  • Stability & Storage
    It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can bestably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstitutedprotein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freezethaw cycles and store products in aliquots
附图

1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 , VEGFA , EGF . Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested)

ed_1793781286408088.png

2.Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.01-0.1 ng/mL

ed_1793781372662214.png

注意事项

1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。

2. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。

3. 实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。

 

备注：由于产品信息可能会有优化升级。请以实际收货标签信息为准。

参考文献

1.Takayama S,et al.(2001) Periodontal regeneration by FGF-2 (bFGF) in primate models. J Dent Res. 80(12): 2075-9.
2.Niu YJ,et al.(2004) Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chin Med J (Engl). 117(2): 252-7.
3.Zhang Y,et al.(2004) Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chin Med J (Engl). 117(4): 601-3.

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