- 生化试剂
- ELISA检测
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抗体蛋白
二抗生物素标记 过氧化物酶(HRP)标记 胶体金试剂 FITC荧光标记 RBITC荧光标记 二抗免疫血清 其它荧光标记二抗 藻红蛋白(PE)荧光标记 胶体金(Gold)标记 SAlexa Fluor荧光系列 碱性磷酸酶(AP)标记 别藻蓝蛋白(APC)荧光标记 其它标记 PE标记二抗 DyLight标记二抗 AU标记二抗 Biotin标记二抗 AMCA标记二抗 Texas Red标记二抗 TRITC标记二抗 HRP标记二抗 未标记二抗 Cy标记二抗 AbBox Fluor标记二抗内参抗体 小分子抗体抗体标记试剂盒细菌抗体蛋白病毒包装试剂杂交瘤融合筛选WB、IHC、ELISA相关试剂细胞培养试剂病原微生物抗原抗体假病毒抗体校准品其他抗原抗体标记的标签抗体病理级IHC抗体重组蛋白
- 细胞培养
- 实验耗材
- 仪器设备
- 生化试剂盒
- 小分子试剂
- 基质胶
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斑马鱼产品
订货时间：周一至周五
订货Q Q：79688691
订货邮件：79688691@qq.com
基本信息-
产品名称异硫氰酸荧光素-D-赖氨酸
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英文名称FITC-D-Lys
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分子式C₂₇H₂₅N₃O₇S
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分子量535.57
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浓度0.1 mM stock solution in anhydrous DMSO
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Ex(nm)488
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Em(nm)520
产品简介1.By the use of the peptidoglycan biosynthetic machinery that metabolic incorporating various non-natural d-amino acids into the peptidoglycan of diverse bacteria. A chemical biology approach that enable rapid and covalent incorporation and detection of a fluorescently derivatized peptidoglycan component during cell wall synthesis in real time, in a wide range of live bacterial species. We employ metabolic incorporation of d-lysine conjugated fluoresceinisocyanate (FITC) into bacterial peptidoglycan for in situ probing live bacteria.
2.Bacterias building its cell wall by the use of d-amino acids so as attache the fluorescein into the cell wall covalently. The probe not only can label gram-negative bacteria but also can label gram-positive bacteria in real time. It can be used for research and exploration infection and pathogenesis of microorganisms.
使用说明(仅供参考)Guidelines for Use
Before opening, allow the vial to warm to room temperature and then briefly centrifuge the vial in amicro centrifuge to deposit the DMSO solution at the bottom of the vial.
The concentration of probe for optimal staining will vary depending on the application. Here we suggest some initial conditions to use as a guideline.The staining conditions may need to be modified depending upon the particular bacteria type to the probe, among other factors.
In vitro staining FITC-d-Lys into E. coli or S. aureus
E. coli or S. aureus were respectively grown at 37℃ in LB medium until OD600 reached 0.6. The medium was diluted to OD600=0.3 with fresh medium containing FITC-d-Lys (Final concentration: 0.1 mM, usually adding 5 μL of stock solution to per mL cell medium). The diluted bacteria were further incubation at 37℃ until OD600=1.0-1.5. The bacteria were centrifuged, washed with LB medium three times, and then resupended in sodium phosphate buffer (100 mM, pH 7.4) or cell culturing medium of interest. The cells were subjected to confocal fluorescence microscopy analysis. (20 mM stock solution of FITC-d-Lys: 10 mg in 0.8 ml water or DMSO)
储存与保存1.保存：≤-20℃，避光保存
2.有效期：12个月
注意事项1.我司生产的生化试剂如无特殊标注，基本为非无菌包装，若用于细胞实验，请提前做好预处理。需低温保存的产品，一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
2.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
3.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。
4.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。
备注：由于产品信息可能会有优化升级，请以实际收货标签信息为准。
