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Rad21 (16H8) Rabbit Monoclonal Antibody  Cite:0    分享

货号： IM68929
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  • 种属：H，M，R
  • 用途：WB，ELISA，IP，IHC-P，IF-P，IF-F，IF-ICC
规格:
50μl 100μl
单位:
支
单价:￥1300.00
产品简介
储存与保存
注意事项
产品简介
  • 产品货号
    IM68929
  • 别名
    RAD21;HR21;KIAA0078;NXP1;Double-strand-break repair protein rad21 homolog;hHR21;Nuclear matrix protein 1;NXP-1;SCC1 homolog
  • 产品名称
    Rad21 (16H8) Rabbit Monoclonal Antibody
  • 类别
    抗体产品
  • 基因名称
    RAD21
  • 蛋白名称
    Double-strand-break repair protein rad21 homolog
  • Clonality
    Monoclonal
  • 推荐应用
    WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
  • 反应种属
    Human,Mouse,Rat
  • 存储缓冲液
    PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
  • Human Gene ID
    5885
  • Human Swissprot No.
    O60216
  • Mouse Gene ID
    19357
  • Mouse Swissprot No.
    Q61550
  • 稀释度
    IHC-P 1:200-1000,WB 1:1000-5000,IF-P/IF-F/IF-ICC 1:200-1000,ELISA 1:5000-20000,IP 1:50-200
  • 参考分子量
    130kDa
  • 预测分子量
    72kDa
  • 宿主
    Rabbit
  • 同种型
    Monoclonal, rabbit, IgG, Kappa
  • 背景介绍
    The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008].
  • 信号通路
    Cell_Cycle_G1S;Cell_Cycle_G2M_DNA.
  • 功能
    domain:The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.,function:Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.,polymorphism:Some radiosensitive cancer patients seem to have Arg-481 instead of the conserved Gly-481. It may be that this mutation could contribute to radiosensitivity.,PTM:Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.,PTM:Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.,similarity:Belongs to the rad21 family.,subcellular location:Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.,subunit:Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Found in a complex with SMC1A, SMC3, CDCA5, PDS5A/APRIN and PDS5B/SCC-112.
  • 纯化
    Protein A
储存与保存

1.保存：-20℃

2.有效期：1年

注意事项

1.我司生产的生化试剂如无特殊标注，基本为非无菌包装，若用于细胞实验，请提前做好预处理。需低温保存的产品，一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

2.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。 

3.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。 

4.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。 

 

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