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PHD2/EGLN1 Rabbit Polyclonal Antibody  Cite:0    分享

货号： IM65841
复制产品信息
  • 种属：H，M，R
  • 用途：WB
规格:
50μl 100μl
单位:
支
单价:￥1200.00
产品简介
储存与保存
注意事项
产品简介
  • 货号
    IM65841
  • 别名
    C1orf12;PNAS-118;PNAS-137
  • 产品名称
    PHD2/EGLN1 Rabbit Polyclonal Antibody
  • 类别
    抗体产品
  • 基因名称
    EGLN1
  • 蛋白名称
    Egl nine homolog 1 (EC 1.14.11.29) (Hypoxia-inducible factor prolyl hydroxylase 2) (HIF-PH2) (HIF-prolyl hydroxylase 2) (HPH-2) (Prolyl hydroxylase domain-containing protein 2) (PHD2) (SM-20)
  • Clonality
    Polyclonal
  • 推荐应用
    WB
  • 反应种属
    Human,Mouse,Rat
  • 浓度
    1 mg/ml
  • 制备方法
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 存储缓冲液
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
  • Human Gene ID
    54583
  • Human Swissprot No.
    Q9GZT9
  • Mouse Gene ID
    112405
  • Mouse Swissprot No.
    Q91YE3
  • Rat Swissprot No.
    P59722
  • 特异性
    This antibody detects endogenous levels of PHD2/EGLN1 at Human, Mouse,Rat
  • 稀释度
    WB 1:500-2000
  • 预测分子量
    47kDa
  • 组织表达
    According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle.
  • 细胞定位
    Cytoplasm . Nucleus . Mainly cytoplasmic. Shuttles between the nucleus and cytoplasm (PubMed:19631610). Nuclear export requires functional XPO1. .
  • 功能
    Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
储存与保存

1.保存：-15~-25℃

2.有效期：1年

注意事项

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