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重组人成纤维生长因子-2/碱性成纤维细胞生长因子 Recombinant Human FGF-2 (bFGF)  Cite:0    分享
Animal-Free，Tag free

货号： F11450
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规格:
20ug 100ug 500ug 1mg
单位:
支
单价:￥720.00
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  • 背景
    Basic fibroblast Growth Factors (FGF-2, bFGF, FGF-β), a 18 kDa pleiotropic cytokine, plays multiple roles in different cells and tissues. FGF-2 can stimulate smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF-2 has been shown to regulate the generation of neurons and astrocytes from progenitor cells. FGF-2 are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. As a multifunctional cytokine, FGF-2 is first isolated from the pituitary. Later, it was identified from various cell types including cardiac myocytes, cardiac fibroblasts, endothelial cells, and smooth muscle cells
  • 别名
    碱性成纤维细胞生长因子 Fibroblast Growth Factors 2, Basic fibroblast Growth Factors , bFGF, Heparin-binding Growth Factors 2 , HBGF-2
  • 蛋白编码
    P09038
  • 分子量
    The protein has a calculated MW of 18.1 kDa. The protein migrates as 15-18 kDa under reducing condition (SDS-PAGE analysis).
  • 表达系统
    Escherichia coli
  • 纯度
    >98% as determined by SDS-PAGE.
  • 生物活性
    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human FGF-2 is approximately >5 x 10⁵ IU/mg.
  • 内毒素检测
    <0.1 EU per 1 μg of the protein by the LAL method.
  • 蛋白序列
    A DNA sequence encoding Human FGF-2 Protein(#P09038) (Ala144-Ser288) was expressed.
  • 蛋白标签
    Tag Free
  • 产品形式
    The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM Tris, 1 M NaCl, pH 7.4. If you have any concerns or special requirements, please confirm with us.
  • 产品应用
    Cell Culture
  • 保存温度
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
  • 产品使用
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
注意事项

1. 开盖前，建议3000-3500rpm离心5min
2. 推荐使用无菌水重悬冻干粉，溶液浓度不低于100μg/mL，不高于1mg/mL，并室温静置至少20min以充分溶解。勿涡旋剧烈振荡
3. 重悬后的溶液，2-8℃无菌保存不超过1周
4. 如需长期保存，推荐使用无菌的含载体蛋白（如0.1%BSA、10%FBS 或5%HSA) 的溶液进一步稀释(不低于10ug/mL) 后分装保存，-20℃至-80℃可无菌保存3到6个月。无血清实验需求时，可更换为 5%海藻糖溶液作为载体。避免反复冻融
5. 本产品仅限于科研使用，不得用于医药、临床诊断或治疗、食品或化妆品等用

6. 请勿存放于普通住宅区

7. 为了您的安全健康，请穿好实验服并佩戴一次性口罩和手套进行操作

8. 实验结果受多种因素影响，相关处理仅限于产品本身，不涉及其他赔偿

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