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GAPDH (2B8) Mouse Monoclonal Antibody  Cite:0    分享

货号： IM12055
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  • 种属：H，M，R，Hm，Mk，B，Dg，Pg，Rab，Ch，Yeast，Insect
  • 用途：WB，IHC-P，IF-ICC
规格:
20μl 50μl 100μl 1ml
单位:
支
单价:￥200.00
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产品详情
  • 产品货号
    IM12055
  • 别名
    GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
  • 产品名称
    GAPDH (2B8) Mouse Monoclonal Antibody
  • 类别
    抗体产品
  • 基因名称
    GAPDH
  • 蛋白名称
    Glyceraldehyde-3-phosphate dehydrogenase
  • 推荐应用
    WB,IF-ICC,IHC-P
  • 反应种属
    human;Rat;Mouse;Mk;Dg;Ch;Hamster;Rabbit;Pig;sheep;Insect;Yeast;Bovine
  • 存储缓冲液
    PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.
  • Human Gene ID
    2597
  • Mouse Gene ID
    100042025
  • Rat Gene ID
    24383
  • 免疫原
    Synthetic Peptide of GAPDH
  • 特异性
    The antibody detects endogenous GAPDH protein.
  • 稀释度
    WB 1:5000-20000, IHC-P 1:200-300, IF-ICC 1:200
  • 参考分子量
    37kD
  • 运输及保存条件
    -20°C/1 year
  • 宿主
    Monoclonal, Mouse
  • 背景介绍
    glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
  • 纯化
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
产品图片
Immunofluorescence analysis of Hela cell. 1,Cyclin D1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.


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1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。
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