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PKAα/β/γ cat (phospho Thr198) Rabbit Polyclonal Antibody  Cite:0    分享

货号： IM50997
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  • 种属：H，M，R
  • 用途：WB，ELISA，IHC-P，IF-P，IF-F，IF-ICC
规格:
50μl 100μl
单位:
支
单价:￥1600.00
产品简介
储存与保存
注意事项
产品简介
  • 产品名称
    PKAα/β/γ cat (phospho Thr198) Rabbit Polyclonal Antibody
  • 别名
    PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma;PRKACB
  • 类别
    抗体产品
  • 基因名称
    PRKACA
  • 蛋白名称
    cAMP-dependent protein kinase catalytic subunit alpha/beta
  • Clonality
    Polyclonal
  • 推荐应用
    WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
  • 反应种属
    Human,Mouse,Rat
  • 浓度
    1 mg/ml
  • 存储缓冲液
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
  • Human Gene ID
    5566/5567
  • Human Swissprot No.
    P17612/P22694/P22612
  • Mouse Gene ID
    18747/18749
  • Rat Gene ID
    293508
  • Rat Swissprot No.
    P27791/P68182
  • 免疫原
    The antiserum was produced against synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197. AA range:166-215
  • 特异性
    Phospho-PKAα/β/γ cat (T198) Polyclonal Antibody detects endogenous levels of PKAα/β/γ cat protein only when phosphorylated at T198.
  • 稀释度
    WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:10000.Not yet tested in other applications.
  • 预测分子量
    40kDa
  • 宿主
    Rabbit
  • 同种型
    Rabbit,IgG
  • 背景介绍
    This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Altern
  • 组织表达
    Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
  • 细胞定位
    Cytoplasm. Cell membrane. Nucleus . Mitochondrion . Membrane ; Lipid-anchor . Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (PubMed:21085490). .; [Isoform 2]: Cell projection, cilium, flagellum . Cytoplasmic vesicle, secretory vesicle, acrosome . Expressed in the midpiece region of the sperm flagellum (PubMed:10906071). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity). .
  • 信号通路
    MAPK_ERK_Growth;MAPK_G_Protein;Calcium;Chemokine;Oocyte meiosis;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Vascular smooth muscle contraction;WNT;WNT-T CELLHedgehog;Gap junction;Long-term potentiation;Olfactory transduction;Taste transduction;Insulin_Receptor;GnRH;Progesterone-mediated oocyte maturation;Melanogenesis;Prion diseases;Vibrio cholerae infection;Dilated cardiomyopathy;
  • 功能
    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by cAMP.,function:Phosphorylates a large number of substrates in the cytoplasm and the nucleus.,PTM:Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.,subunit:A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.,tissue specificity:Isoform 2 is sperm specific.,
  • 纯化
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存与保存

1. 保存：-20℃，Avoid freeze/thaw cycles

2. 有效期：1年

注意事项

1. 我司生产的生化试剂如无特殊标注，基本为非无菌包装，若用于细胞实验，请提前做好预处理。需低温保存的产品，一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

2. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。 

3. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。 

4. 实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。 

 

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