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EGF Protein, Human, Recombinant  Cite:20    分享
重组蛋白满5送1  

货号： PR0001
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单价:￥190.00
基本信息
产品简介
储存与保存
注意事项
基本信息
  • 产品名称
    EGF Protein, Human, Recombinant
  • 别名
    表皮生长因子, URG, HOMG4, epidermal growth factor
  • 种属
    Human
  • 表达系统
    E. coli
  • 标签
    Tag Free
  • 蛋白编号
    P01133-1
  • 蛋白构建
    A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn971-Arg1023) was expressed and purified with an initial Met at the N-terminus. Predicted N terminal: Met
  • 蛋白纯度
    ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
  • 蛋白性状
    Lyophilized powder
  • 分子量
    6.35 kDa (predicted); 6.35 kDa (reducing conditions)
  • 缓冲液
    Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
  • 复溶方法
    Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
  • 内毒素
    < 5 EU/mg of the protein
  • 生物活性
    1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 , VEGFA , EGF . Red arrows represent vascular organoids. Image taken at 10x magnification.(Routinely tested) 2.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml.
  • 研究背景
    EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.
  • 运输条件
    In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
产品简介

EGF Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 6.35 kDa and the accession number is P01133-1.

储存与保存

It is recommended to store recombinant proteins at -20°C to -80°C for future use.
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

注意事项

1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。

2. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。

3. 实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。

 

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